To Process a Generic 1-H Spectrum:
Open the file containing the spectrum.
If your spectrum corresponds to a folder, choose any of the files at the top level of that folder. iNMR will then show the raw, time-domain, data.
On the Mac, you can alternatively drag the folder containing the spectrum onto the iNMR icon.
Fourier Transform.
Choose the command Process > FT now.
On the Mac it appears when you hold down the alt key. If you prefer custom processing, choose, instead, Process > Fourier Transform. It opens a dialog for setting zero-filling, weighting and other options.
Adjust the amplification, if necessary.
A double click inside the plot area changes the amplification so that the the tallest peak fits exactly into the window. The plus and minus keys have an intuitive effect on amplification.
Correct the Phase.
iNMR has already applied a rough automatic correction. Should it be not enough, either choose Process > Automatic Phase or correct the phase manually.
Correct the Baseline.
Choose Process > Baseline Correction and click OK or, to make things faster, click the corresponding icon (red signal with a gear).
Calibrate the Frequency axis.
Right-click the reference peak, choose Reference Scale and enter the correct ppm value.
Define the Integral Regions.
Automatically: choose View > Auto-Integrate.
With the mouse: pick the Integrator tool and drag over each multiplet.
Without the tool: select each region with the mouse and press “i” on the keyboard.
To normalize the numerical values:
Pick the integrator tool; double click an integral; enter the normalized value.
Peak the Peaks.
Select the peak-picker tool, click above the noise level but below the peaks height and drag over the regions of interest.
Extract shifts and couplings.
Choose View > J couplings to open the madule called J Manager; select a multiplet and press “e” on the keyboard. To generate a formatted list, click the icon report, inside the J Manager.